Details

Autor(en) / Beteiligte

• Dagrosa, Maria Alejandra, Ph.D
• Crivello, Martín, M.Sc
• Perona, Marina, M.Sc
• Thorp, Silvia, M.Sc
• Santa Cruz, Gustavo Alberto, Ph.D
• Pozzi, Emiliano, M.Sc
• Casal, Mariana, M.Sc
• Thomasz, Lisa, Ph.D
• Cabrini, Romulo, M.D
• Kahl, Steven, Ph.D
• Juvenal, Guillermo Juan, Ph.D
• Pisarev, Mario Alberto, M.D

Titel

First Evaluation of the Biologic Effectiveness Factors of Boron Neutron Capture Therapy (BNCT) in a Human Colon Carcinoma Cell Line

Ist Teil von

• International journal of radiation oncology, biology, physics, 2011, Vol.79 (1), p.262-268

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Purpose DNA lesions produced by boron neutron capture therapy (BNCT) and those produced by gamma radiation in a colon carcinoma cell line were analyzed. We have also derived the relative biologic effectiveness factor (RBE) of the neutron beam of the RA-3- Argentine nuclear reactor, and the compound biologic effectiveness (CBE) values for p-boronophenylalanine (10 BPA) and for 2,4-bis (α,β-dihydroxyethyl)-deutero-porphyrin IX (10 BOPP). Methods and Materials Exponentially growing human colon carcinoma cells (ARO81-1) were distributed into the following groups: (1) BPA (10 ppm10 B) + neutrons, (2) BOPP (10 ppm10 B) + neutrons, (3) neutrons alone, and (4) gamma rays (60 Co source at 1 Gy/min dose-rate). Different irradiation times were used to obtain total absorbed doses between 0.3 and 5 Gy (±10%) (thermal neutrons flux = 7.5 109 n/cm2 sec). Results The frequency of micronucleated binucleated cells and the number of micronuclei per micronucleated binucleated cells showed a dose-dependent increase until approximately 2 Gy. The response to gamma rays was significantly lower than the response to the other treatments ( p < 0.05). The irradiations with neutrons alone and neutrons + BOPP showed curves that did not differ significantly from, and showed less DNA damage than, irradiation with neutrons + BPA. A decrease in the surviving fraction measured by 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromide (MTT) assay as a function of the absorbed dose was observed for all the treatments. The RBE and CBE factors calculated from cytokinesis block micronucleus (CBMN) and MTT assays were, respectively, the following: beam RBE: 4.4 ± 1.1 and 2.4 ± 0.6; CBE for BOPP: 8.0 ± 2.2 and 2.0 ± 1; CBE for BPA: 19.6 ± 3.7 and 3.5 ± 1.3. Conclusions BNCT and gamma irradiations showed different genotoxic patterns. To our knowledge, these values represent the first experimental ones obtained for the RA-3 in a biologic model and could be useful for future experimental studies for the application of BNCT to colon carcinoma.