Details

Autor(en) / Beteiligte

• Liu, Xiangde
• Nelson, Amy
• Wang, Xingqi
• Kanaji, Nobuhiro
• Kim, Miok
• Sato, Tadashi
• Nakanishi, Masanori
• Li, YingJi
• Sun, Jianhong
• Michalski, Joel
• Patil, Amol
• Basma, Hesham
• Rennard, Stephen I.

Titel

MicroRNA-146a modulates human bronchial epithelial cell survival in response to the cytokine-induced apoptosis

Ist Teil von

• Biochemical and biophysical research communications, 2009-02, Vol.380 (1), p.177-182

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2009

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• MicroRNA plays an important role in cell differentiation, proliferation and cell death. The current study found that miRNA-146a was up-regulated in human bronchial epithelial cells (HBECs) in response to stimulation by TGF-ß1 plus cytomix (a mixture of IL-1ß, IFN-γ and TNF-α). TGF-ß1 plus cytomix (TCM) induced apoptosis in HBECs (3.4 ± 0.6% of control vs 83.1 ± 4.0% of TCM treated cells, p < 0.01), and this was significantly blocked by the miRNA-146a mimic (8.8 ± 1.5%, p < 0.01). In contrast, a miRNA-146a inhibitor had only a modest effect on cell survival but appeared to augment the induction of epithelial-mesenchymal transition (EMT) in response to the cytokines. The MicroRNA-146a mimic appears to modulate HBEC survival through a mechanism of up-regulating Bcl-XL and STAT3 phosphorylation, and by this mechanism it could contribute to tissue repair and remodeling.