Details

Autor(en) / Beteiligte

• Schabort, Elske J
• van der Merwe, Mathilde
• Loos, Benjamin
• Moore, Frances P
• Niesler, Carola U

Titel

TGF-[beta]'s delay skeletal muscle progenitor cell differentiation in an isoform-independent manner

Ist Teil von

• Experimental cell research, 2009-02, Vol.315 (3), p.373

Ort / Verlag

New York: Elsevier BV

Erscheinungsjahr

2009

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Satellite cells are a quiescent heterogenous population of mononuclear stem and progenitor cells which, once activated, differentiate into myotubes and facilitate skeletal muscle repair or growth. The Transforming Growth Factor-[beta] (TGF-[beta]) superfamily members are elevated post-injury and their importance in the regulation of myogenesis and wound healing has been demonstrated both in vitro and in vivo. Most studies suggest a negative role for TGF-[beta] on satellite cell differentiation. However, none have compared the effect of these three isoforms on myogenesis in vitro. This is despite known isoform-specific effects of TGF-[beta]1, -[beta]2 and -[beta]3 on wound repair in other tissues. In the current study we compared the effect of TGF-[beta]1, -[beta]2 and -[beta]3 on proliferation and differentiation of the C2C12 myoblast cell-line. We found that, irrespective of the isoform, TGF-[beta] increased proliferation of C2C12 cells by changing the cellular localisation of PCNA to promote cell division and prevent cell cycle exit. Concomitantly, TGF-[beta]1, -[beta]2 and -[beta]3 delayed myogenic commitment by increasing MyoD degradation and decreasing myogenin expression. Terminal differentiation, as measured by a decrease in myosin heavy chain (MHC) expression, was also delayed. These results demonstrate that TGF-[beta] promotes proliferation and delays differentiation of C2C12 myoblasts in an isoform-independent manner. [PUBLICATION ABSTRACT]