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Validation of in vitro cell models used in drug metabolism and transport studies; genotyping of cytochrome P450, phase II enzymes and drug transporter polymorphisms in the human hepatoma (HepG2), ovarian carcinoma (IGROV-1) and colon carcinoma (CaCo-2, LS180) cell lines
Toxicology and applied pharmacology, 2006-02, Vol.211 (1), p.1-10
2006

Details

Autor(en) / Beteiligte
Titel
Validation of in vitro cell models used in drug metabolism and transport studies; genotyping of cytochrome P450, phase II enzymes and drug transporter polymorphisms in the human hepatoma (HepG2), ovarian carcinoma (IGROV-1) and colon carcinoma (CaCo-2, LS180) cell lines
Ist Teil von
  • Toxicology and applied pharmacology, 2006-02, Vol.211 (1), p.1-10
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
2006
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
  • Human cell lines are often used for in vitro biotransformation and transport studies of drugs. In vivo, genetic polymorphisms have been identified in drug-metabolizing enzymes and ABC-drug transporters leading to altered enzyme activity, or a change in the inducibility of these enzymes. These genetic polymorphisms could also influence the outcome of studies using human cell lines. Therefore, the aim of our study was to pharmacogenotype four cell lines frequently used in drug metabolism and transport studies, HepG2, IGROV-1, CaCo-2 and LS180, for genetic polymorphisms in biotransformation enzymes and drug transporters. The results indicate that, despite the presence of some genetic polymorphisms, no real effects influencing the activity of metabolizing enzymes or drug transporters in the investigated cell lines are expected. However, this characterization will be an aid in the interpretation of the results of biotransformation and transport studies using these in vitro cell models.
Sprache
Englisch
Identifikatoren
ISSN: 0041-008X
eISSN: 1096-0333
DOI: 10.1016/j.taap.2005.05.004
Titel-ID: cdi_osti_scitechconnect_20783424
Format
–
Schlagworte
60 APPLIED LIFE SCIENCES, ANIMAL CELLS, ATP-Binding Cassette, Sub-Family B, Member 1 - genetics, ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism, Biological Transport, Biotransformation, CaCo-2, Caco-2 Cells, Carcinoma - enzymology, Carcinoma - metabolism, Carcinoma, Hepatocellular - enzymology, Carcinoma, Hepatocellular - metabolism, Cell Line, Tumor, Colonic Neoplasms - enzymology, Colonic Neoplasms - metabolism, Cytochrome P-450 Enzyme System - genetics, Cytochrome P-450 Enzyme System - metabolism, Drug Screening Assays, Antitumor - methods, Drug Screening Assays, Antitumor - standards, Drug transporters, DRUGS, ENZYME ACTIVITY, ENZYMES, Female, Genetic polymorphisms, HEPATOMAS, HepG2, Humans, IGROV-1, IN VITRO, IN VIVO, Inactivation, Metabolic - genetics, LARGE INTESTINE, Liver Neoplasms - enzymology, Liver Neoplasms - metabolism, LS180 cytochrome P450, Membrane Transport Proteins - genetics, Membrane Transport Proteins - metabolism, METABOLISM, Ovarian Neoplasms - enzymology, Ovarian Neoplasms - genetics, Pharmacogenetics - methods, Phase II enzymes, Polymorphism, Genetic, Transferases - genetics, Transferases - metabolism, VALIDATION

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