Details

Autor(en) / Beteiligte

• Sandoval, Braddock A.
• Clayman, Phillip D.
• Oblinsky, Daniel G.
• Oh, Seokjoon
• Nakano, Yuji
• Bird, Matthew
• Scholes, Gregory D.
• Hyster, Todd K.

Titel

Photoenzymatic Reductions Enabled by Direct Excitation of Flavin-Dependent “Ene”-Reductases

Ist Teil von

• Journal of the American Chemical Society, 2020-12, Vol.143 (4)

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2020

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Non-natural photoenzymatic reactions reported to date have depended on the excitation of electron donor–acceptor complexes formed between substrates and cofactors within protein active sites to facilitate electron transfer. While this mechanism has unlocked new reactivity, it limits the types of substrates that can be involved in this area of catalysis. Here we demonstrate that direct excitation of flavin hydroquinone within “ene”-reductase active sites enables new substrates to participate in photoenzymatic reactions. We found that by using photoexcitation these enzymes gain the ability to reduce acrylamides through a single electron transfer mechanism.