Details

Autor(en) / Beteiligte

• Romine, Margaret F
• Rodionov, Dmitry A
• Maezato, Yukari
• Anderson, Lindsey N
• Nandhikonda, Premchendar
• Rodionova, Irina A
• Carre, Alexandre
• Li, Xiaoqing
• Xu, Chengdong
• Clauss, Therese R W
• Kim, Young-Mo
• Metz, Thomas O
• Wright, Aaron T

Titel

Elucidation of roles for vitamin B 12 in regulation of folate, ubiquinone, and methionine metabolism

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2017-02, Vol.114 (7), p.E1205

Ort / Verlag

United States: Proceedings of the National Academy of Sciences

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Only a small fraction of vitamin B -requiring organisms are able to synthesize B de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B -binding enzymes and regulatory roles for B Here we report the development and use of a B -based chemical probe to identify B -binding proteins in a nonphototrophic B -producing bacterium. Two unexpected discoveries resulted from this study. First, we identified a light-sensing B -binding transcriptional regulator and demonstrated that it controls folate and ubiquinone biosynthesis. Second, our probe captured proteins involved in folate, methionine, and ubiquinone metabolism, suggesting that it may play a role as an allosteric effector of these processes. These metabolic processes produce precursors for synthesis of DNA, RNA, and protein. Thereby, B likely modulates growth, and by limiting its availability to auxotrophs, B -producing organisms may facilitate coordination of community metabolism.