Talanta (Oxford), 2012-05, Vol.94, p.58-64
2012
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- Autor(en) / Beteiligte
- Titel
- An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers
- Ist Teil von
- Talanta (Oxford), 2012-05, Vol.94, p.58-64
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2012
- Quelle
- ScienceDirect
- Beschreibungen/Notizen
- ► A test strip based biosensor for rapid screening of HBsAg is proposed. ► Enzymatic electrochemical measurement allows sensitive and quantitative detection. ► The enzyme label simplifies the fabrication of test strip and detection procedure. A novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (SPE) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. The principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (SWV) detection. Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. Hepatitis B surface antigen (HBsAg) was employed as a model protein biomarker to demonstrate the analytical performance of the sensor in this study. Some critical parameters governing the performance of the sensor were investigated in detail. Under optimal conditions, this sensor was capable of detecting a minimum of 0.3ngmL−1 (S/N=3) HBsAg with a wide linear concentration range from 1 to 500ngmL−1. The sensor was further utilized to detect HBsAg spiked in human plasma with an average recovery of 91.3%. In comparison, a colorimetric immunochromatographic test strip assay (ITSA) was also conducted. The result shows that the SWV detection in the electrochemical sensor is much more sensitive for the quantitative determination of HBsAg than the colorimetric detection, indicating that such a sensor is a promising platform for rapid and sensitive point-of-care testing/screening of disease-related biomarkers in a large population.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0039-9140eISSN: 1873-3573DOI: 10.1016/j.talanta.2012.02.046Titel-ID: cdi_osti_scitechconnect_1044488
- Format
- –
- Schlagworte
- 60 APPLIED LIFE SCIENCES, Analytical chemistry, ANTIBODIES, Antibodies - chemistry, Antibodies - immunology, ANTIGENS, BASIC BIOLOGICAL SCIENCES, Biological and medical sciences, biomarkers, Biomarkers - blood, Biosensing Techniques - methods, Biosensors, Biotechnology, BLOOD, Chemistry, DETECTION, Disease-related biomarker, Electrochemical methods, Electrochemical Techniques, ELECTRODES, ENZYMES, Equipment Design, Exact sciences and technology, Fundamental and applied biological sciences. Psychology, General, instrumentation, HEPATITIS, Hepatitis B surface antigen, Hepatitis B Surface Antigens - blood, Horseradish peroxidase, Horseradish Peroxidase - chemistry, Humans, immunoassay, Immunochromatographic test strip assay, Immunochromatography - methods, Limit of Detection, Methods. Procedures. Technologies, PEROXIDASES, PLASMA, PROTEINS, Quantum Dots, Reagent Strips - chemistry, SENSORS, SOLAR PROTONS, Spectrometric and optical methods, Staining and Labeling, Various methods and equipments