Details

Autor(en) / Beteiligte

• Rahman, Md Atikur
• Alam, Iftekhar
• Sharmin, Shamima Akhtar
• Kabir, Ahmad Humayan
• Kim, Yong-Goo
• Liu, Gongshe
• Lee, Byung-Hyun

Titel

Physiological and proteomic analyses reveal the protective roles of exogenous hydrogen peroxide in alleviating drought stress in soybean plants

Ist Teil von

• Plant Biotechnology Reports, 2021, 15(6), , pp.805-818

Ort / Verlag

Singapore: Springer Singapore

Erscheinungsjahr

2021

Quelle

SpringerLink

Beschreibungen/Notizen

• Drought stress is one of the major constraints for soybean growth and productivity worldwide. The study was aimed to investigate drought-induced physiological and proteomic changes in soybeans, as well as drought relief using exogenous hydrogen peroxide (H 2 O 2 ). In drought-stressed plants, H 2 O 2 spray on the leaf surface improved relative water content (RWC), net photosynthetic rate (Pn), and stomatal conductance (Gs). Furthermore, exogenous H 2 O 2 reduced drought stress-induced endogenous MDA and H 2 O 2 levels, as well as increased the key antioxidant enzymes (SOD,CAT, APX and POD) activity and proline content in H 2 O 2 -treated soybean plants. These findings showed that H 2 O 2 treatment significantly reduced drought stress by increasing the antioxidative defense system and osmotic adjustment. Furthermore, using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, a total of 27 differently expressed proteins was identified, wherein 23 were up-regulated and 4 were down-regulated under drought condition. These proteins were found to be involved in photosynthesis, energy and metabolism, plant defense and antioxidant, signaling and transport, and transcription regulation in response to H 2 O 2 treatment in soybean under drought stress, according to in silico interactome analysis. These findings add to our understanding of H 2 O 2 -mediated drought stress alleviation, as well as the physiological and molecular responses of soybean to drought stress.