Details

Autor(en) / Beteiligte

• Xianjing Zheng
• Haimei Liu
• Maoqiang Ma
• Jianbo Ji
• Faliang Zhu
• Longru Sun

Titel

Anti-thrombotic activity of phenolic acids obtained from Salvia miltiorrhiza f. alba in TNF-a-stimulated endothelial cells via the NF-kB/JNK/p38 MAPK signaling pathway

Ist Teil von

• Archives of Pharmacal Research, 2021, 44(4), , pp.427-438

Ort / Verlag

대한약학회

Erscheinungsjahr

2021

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Over the past 100 years, Salvia miltiorrhiza f. alba (Lamiaceae) (RSMA) roots have been used to curethromboangiitis obliterans (TAO) in local clinics. This studyaimed to confi rm the anti-thrombotic effi cacy of 12 phenolicacids obtained from RSMA and to clarify the possibleunderlying mechanisms. The results of quantitative real-timepolymerase chain reaction (qRT-PCR) and enzyme-linkedimmunosorbent assay (ELISA) experiments demonstratedthat most of the phenolic acids markedly inhibited PAI-1protein and mRNA levels but increased t-PA protein andmRNA levels in TNF-α-induced EA.hy926 cells ( P < 0.05or 0.001), with lithospermic acid displaying the strongesteff ect. In vitro anticoagulation and antiplatelet aggregationassays showed that lithospermic acid and salvianolic acidB signifi cantly prolonged prothrombin time (PT), activatedpartial thromboplastin time (APTT), decreased fi brinogen concentration (FIB), and inhibited platelet aggregationinduced by adenosine diphosphate (ADP) in rat blood. Bothlithospermic acid and salvianolic acid B markedly down-regulatedthe expression of factor Xa and factor IIa on the externalsurface of EA.hy926 cells and demonstrated signifi cantanti-factor IIa and anti-factor Xa activity using chromogenicsubstrates in vitro. Western blot results revealed that bothlithospermic acid and salvianolic acid B also signifi cantlyinhibited the expression of TF, p-p65, p-p38, and pJNK proteinsinduced by TNF-α. These results indicated that all ofthe phenolic acids appeared to have some anti-thromboticactivity, with salvianolic acid B and lithospermic acid markedlydecreasing the chance of thrombosis by regulating theNF-κB/JNK/p38 MAPK signaling pathway in response toTNF-α. KCI Citation Count: 3