Details

Autor(en) / Beteiligte

• Yang, Kang
• Wang, Na
• Guo, Hai-Tao
• Wang, Jing-Ran
• Sun, Huan-Huan
• Sun, Liang-Zhen
• Yue, Shun-Li
• Zhou, Jia-Bo

Titel

Effect of L-carnitine on sperm quality during liquid storage of boar semen

Ist Teil von

• Animal Bioscience, 2020, 33(11), , pp.1763-1769

Ort / Verlag

Korea (South): Asian - Australasian Association of Animal Production Societies

Erscheinungsjahr

2020

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• This study was to investigate the effect of L-carnitine on the characteristics in fresh semen storage of pig. Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 mM (control), 12.5 mM, 25 mM, 50 mM and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (t-ROS, T-AOC and MDA) were evaluated after 0, 3, 5, and 10 day cooled-storage at 17 ℃. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated. Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida (ZP), without improving in vitro fertility and development potential. These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17℃.