Details

Autor(en) / Beteiligte

• Hyun, C.G. (Chem Tech Research Incorporation (C-TRI), Kowoon Institute of Technology Innovation, Whasung, Republic of Korea)
• Kim, S.Y. (Chem Tech Research Incorporation (C-TRI), Kowoon Institute of Technology Innovation, Whasung, Republic of Korea)
• Hur, J.H. (Chem Tech Research Incorporation (C-TRI), Kowoon Institute of Technology Innovation, Whasung, Republic of Korea)
• Seo, M.J. (Chem Tech Research Incorporation (C-TRI), Kowoon Institute of Technology Innovation, Whasung, Republic of Korea)
• Suh, J.W. (Myongji University, Yongin, Republic of Korea)
• Kim, S.O. (Chem Tech Research Incorporation (C-TRI), Kowoon Institute of Technology Innovation, Whasung, Republic of Korea), E-mail: sokim@c-tri.com

Titel

Molecular Detection of α-Glucosidase Inhibitor-producing Actinomycetes

Ist Teil von

• The Journal of Microbiology, 2005, 43(3), , pp.313-318

Ort / Verlag

Korea (South): 한국미생물학회

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• In this study, we demonstrate the use of a PCR-based method for the detection of the specific genes involved in natural-product biosynthesis. This method was applied, using specifically designed PCR primers, to the amplification of a gene segment encoding for sedo-heptulose 7-phosphate cyclase, which appears to be involved in the biosynthetic pathways of C∧7N aminoacyclitol or its keto analogue-containing metabolites, in a variety of actinomycetes species. The sequences of DNA fragments (about 540 bp) obtained from three out of 39 actinomycete strains exhibited a high degree of homology with the sedo-heptulose 7-phosphate cyclase gene, which has been implicated in acarbose biosynthesis.