Details

Autor(en) / Beteiligte

• Cha, S.H. (Inha University, Incheon, Republic of Korea), E-mail: shcha@inha.ac.kr
• Park, J.E.
• Kim, J.B. (Catholic University of Daegu, Gyeongsan, Republic of Korea)
• Lee, K.Y. (Yeungnam University, Daegu, Republic of Korea)
• Kwak, J.O. (Inha University, Incheon, Republic of Korea)
• Kim, H.W. (Inha University, Incheon, Republic of Korea)
• Cha, Y.N. (Inha University, Incheon, Republic of Korea)

Titel

Attenuation of Extracellular Acidic pH-induced Cyclooxygenase-2 Expression by Nitric Oxide

Ist Teil von

• Molecules and Cells, 2005, 19(2), , pp.232-238

Ort / Verlag

United States: 한국분자세포생물학회

Erscheinungsjahr

2005

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• Corneal endothelial cells play an important role in maintaining the transparency and ionic balance of the cornea. Inflammation causes many changes in the intracellular and extracellular environment of the cornea, including acidosis. We examined the relationship between changes in extracellular pH and expression of cyclooxygenase-2 in cultured bovine corneal endothelial cells. When extracellular pH ([pH]∧o) was reduced to pH 6.4, COX-2 mRNA increased, with a peak at 2 h. This was blocked by pretreatment with actinomycin D and incubation with spermine NONOate (SPER/NO, a nitric oxide donor). Exposure to the H+ ionophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP), also raised COX-2 mRNA levels.