Details

Autor(en) / Beteiligte

• Chung, I.H., Hanyang University and GenoCheck Co. Ltd., Ansan, Republic of Korea
• Yoo, H.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
• Eah, J.Y., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
• Yoon, H.K., Hanyang University and GenoCheck Co. Ltd., Ansan, Republic of Korea
• Jung, J.W., Hanyang University and GenoCheck Co. Ltd., Ansan, Republic of Korea
• Hwang, S.Y., Hanyang University and GenoCheck Co. Ltd., Ansan, Republic of Korea
• Kim, C.B., Sangmyung University, Seoul, Republic of Korea

Titel

A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences

Ist Teil von

• Molecules and Cells, 2010, 30(4), , pp.295-301

Ort / Verlag

Springer: Korean Society for Molecular and Cellular Biology

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.