Details

Autor(en) / Beteiligte

• Han, M.J. (Dongguk University, Seoul, Republic of Korea)
• Seo, Y.K. (Dongguk University, Seoul, Republic of Korea)
• Yoon, H.H. (Dongguk University, Seoul, Republic of Korea)
• Song, K.Y. (Chung-Ang University, Seoul, Republic of Korea)
• Park, J.K. (Dongguk University, Seoul, Republic of Korea), E-mail: jkpark@dongguk.edu

Titel

Effect of mechanical tension on the human dental pulp cells

Ist Teil von

• Biotechnology and Bioprocess Engineering, 2008, 13(4), , pp.410-417

Ort / Verlag

Heidelberg: The Korean Society for Biotechnology and Bioengineering

Erscheinungsjahr

2008

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• In this study, we have evaluated the effects of mechanical tension on the proliferation and extracellular matrix (ECM) production of human dental pulp stem cells (DPSCs) using a flexwell system that imposed cyclic mechanical tension at 0.03 Hz with 0, 5, and 8% strains. In the early stage (4 days), DPSCs at 5 and 8% strains had a similar proliferation, which was higher than the control. However, in the late stage (10 days), DPSCs at 8% strain had a higher proliferation than the control and 5% strains. This result clearly demonstrated that DPSC proliferation under tension varied with culture time. In addition, mechanical tension was shown to increase the amount of lactate dehydrogenase (LDH) released during culture. RT-PCR analysis was used to show that mechanical tension also increased collagen and osteopontin expression and decreased alpha-smooth muscle actin (α-SMA) expression. Furthermore, FACS analysis showed that CD105 expression did not change in all groups but CD 90 expression decreased at 8% strain. In conclusion, our results suggest that an appropriate level of mechanical tension can serve as a potent positive modulator of proliferation, differentiation and ECM production in DPSCs.