Details
- Autor(en) / Beteiligte
- Titel
- Mind Bomb-Binding Partner RanBP9 Plays a Contributory Role in Retinal Development
- Ist Teil von
- Molecules and Cells, 2017, 40(4), , pp.271-279
- Ort / Verlag
- United States: Korean Society for Molecular and Cellular Biology
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown of resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation marker , elevation of neural proliferation marker , and alteration of cell cycle marker . Expression of the Müller glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bomb-binding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1016-8478eISSN: 0219-1032DOI: 10.14348/molcells.2017.2308Titel-ID: cdi_nrf_kci_oai_kci_go_kr_ARTI_1373252
- Format
- –
- Schlagworte
- Adaptor Proteins, Signal Transducing - genetics, Adaptor Proteins, Signal Transducing - metabolism, Animals, Basic Helix-Loop-Helix Transcription Factors - genetics, Basic Helix-Loop-Helix Transcription Factors - metabolism, Brain - cytology, Brain - embryology, Brain - metabolism, Cell Proliferation, Chlorocebus aethiops, COS Cells, Cyclin-Dependent Kinase Inhibitor p57 - genetics, Cyclin-Dependent Kinase Inhibitor p57 - metabolism, Cytoskeletal Proteins - genetics, Cytoskeletal Proteins - metabolism, Down-Regulation, ELAV-Like Protein 3 - genetics, ELAV-Like Protein 3 - metabolism, Ependymoglial Cells - physiology, Gene Knockdown Techniques, Neurogenesis - physiology, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Retina - cytology, Retina - embryology, Retina - metabolism, Retinal Dysplasia - genetics, Two-Hybrid System Techniques, Ubiquitin-Protein Ligases - genetics, Ubiquitin-Protein Ligases - metabolism, Zebrafish - embryology, Zebrafish - genetics, Zebrafish - metabolism, Zebrafish Proteins - genetics, Zebrafish Proteins - metabolism, 생물학