Details

Autor(en) / Beteiligte

• Kimura Soichiro
• Morimoto Keiko
• Okamoto Hiroshi
• Ueda Hideo
• Kobayashi Daisuke
• Kobayashi Jun
• Morimoto Yasunori

Titel

Development of a Human Mammary Epithelial Cell Culture Model for Evaluation of Drug Transfer into Milk

Ist Teil von

• Archives of Pharmacal Research, 2006, 29(5), , pp.424-429

Ort / Verlag

대한약학회

Erscheinungsjahr

2006

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• In the present study, a human mammary epithelial cell (HMEC) culture model was developed to evaluate the potential involvement of carrier-mediated transport systems in drug transfer into milk. Trypsin-resistant HMECs were seeded on $Matrigel^{circledR}-coated$ filters to develop monolayers of functionally differentiated HMEC. Expression of the specific function of HMEC monolayers was dependent of the number of trypsin treatments. Among the monolayers with different numbers of treatment (treated 1 to 3 times), the monolayer treated 3 times (3-t-HMEC monolayer) showed the highest maximal transepithelial resistance and expression of $\beta-casein$ mRNA as an index of differentiation. Transport of tetraethylammonium (TEA) across the 3-t-HMEC monolayer in the basolateral-to-apical direction was significantly higher than that in the apical-to-basolateral direction (p<0.05), whereas such directionality was not observed for p-aminohippurate, suggesting the existence of organic cation transporters, but not organic anion transporters. In fact, expression of mRNAs of human organic cation transporter (OCT) 1 and 3 were detected in the 3-t-HMEC monolayer. These results indicate that the 3-t-HMEC monolayer is potentially useful for the evaluation of carrier-mediated secretion of drugs including organic cations into human milk.