BMB Reports, 2013, 46(2), , pp.107-112
- Zhang, Y., Chongqing Medical University, Chongqing, PR China
- Zhang, D.Y., Chongqing Medical University, Chongqing, PR China
- Zhao, Y.F., Chongqing Medical University, Chongqing, PR China
- Wang, J., Chongqing Medical University, Chongqing, PR China
- He, J.W., Chongqing Medical University, Chongqing, PR China
- Lou, J.Y., Chongqing Medical University, Chongqing, PR China
2013
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- Autor(en) / Beteiligte
- Zhang, Y., Chongqing Medical University, Chongqing, PR China
- Zhang, D.Y., Chongqing Medical University, Chongqing, PR China
- Zhao, Y.F., Chongqing Medical University, Chongqing, PR China
- Wang, J., Chongqing Medical University, Chongqing, PR China
- He, J.W., Chongqing Medical University, Chongqing, PR China
- Lou, J.Y., Chongqing Medical University, Chongqing, PR China
- Titel
- Analysis and characterization of the functional TGFβ receptors required for BMP6-induced osteogenic differentiation of mesenchymal progenitor cells
- Ist Teil von
- BMB Reports, 2013, 46(2), , pp.107-112
- Ort / Verlag
- Korea (South): Korean Society for Biochemistry and Molecular Biology
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Although BMP6 is highly capable of inducing osteogenic differentiation of mesenchymal progenitor cells (MPCs), the molecular mechanism involved remains to be fully elucidated. Using dominant negative (dn) mutant form of type I and type II TGFβ receptors, we demonstrated that three dn-type I receptors (dnALK2, dnALK3, dnALK6), and three dn-type II receptors (dnBMPRII, dnActRII, dnActRIIB), effectively diminished BMP6- induced osteogenic differentiation of MPCs. These findings suggested that ALK2, ALK3, ALK6, BMPRII, ActRII and ActRIIB are essential for BMP6-induced osteogenic differentiation of MPCs. However, MPCs in this study do not express ActRIIB. Moreover, RNA interference of ALK2, ALK3, ALK6, BMPRII and ActRII inhibited BMP6-induced osteogenic differentiation in MPCs. Our results strongly suggested that BMP6-induced osteogenic differentiation of MPCs is mediated by its functional TGFβ receptors including ALK2, ALK3, ALK6, BMPRII, and ActRII.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1976-6696eISSN: 1976-670XDOI: 10.5483/BMBRep.2013.46.2.141Titel-ID: cdi_nrf_kci_oai_kci_go_kr_ARTI_1088429
- Format
- –
- Schlagworte
- Activin Receptors, Type I - antagonists & inhibitors, Activin Receptors, Type I - genetics, Activin Receptors, Type I - metabolism, Activin Receptors, Type II - antagonists & inhibitors, Activin Receptors, Type II - genetics, Activin Receptors, Type II - metabolism, Animals, BMP6, Bone Morphogenetic Protein 6 - genetics, Bone Morphogenetic Protein 6 - metabolism, Bone Morphogenetic Protein Receptors, Type I - antagonists & inhibitors, Bone Morphogenetic Protein Receptors, Type I - genetics, Bone Morphogenetic Protein Receptors, Type I - metabolism, Bone Morphogenetic Protein Receptors, Type II - antagonists & inhibitors, Bone Morphogenetic Protein Receptors, Type II - genetics, Bone Morphogenetic Protein Receptors, Type II - metabolism, Bone morphogenetic proteins, Cell Differentiation, Cell Line, CELLS, CELLULE, CELULAS, HEK293 Cells, Humans, Mesenchymal progenitor cells, Mesenchymal Stem Cells - cytology, Mesenchymal Stem Cells - metabolism, Mice, Osteogenesis, Osteogenic differentiation, Osteogenic differentiation,Transforming growth factor beta receptor,Mesenchymal progenitor cell, Receptors, Transforming Growth Factor beta - metabolism, RNA Interference, RNA, Small Interfering - metabolism, Signal Transduction, Smad Proteins - metabolism, Transfection, Transforming growth factor β receptor, 화학