Details

Autor(en) / Beteiligte

• Hwang, Chi Young
• Cho, Eui-Sang
• Rhee, Won Jong
• Kim, Eunjung
• Seo, Myung-Ji

Titel

Genomic and physiological analysis of C50 carotenoid-producing novel Halorubrum ruber sp. nov

Ist Teil von

• The Journal of Microbiology, 2022, 60(10), , pp.1007-1020

Ort / Verlag

Seoul: The Microbiological Society of Korea

Erscheinungsjahr

2022

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• A novel haloarchaeal species designated as MBLA0099 T was isolated from seawater near Yeongheung Island. Cells were Gram-negative, non-motile, red-pigmented, and rod-shaped. They grew at 10–45°C, within pH 5.5–9.0, and between 7.5% and 30% NaCl concentrations. Cells were able to grow without Mg 2+ and were lysed in distilled water. The size of the whole-genome and G + C content of DNA was 3.02 Mb and 68.9 mol%, respectively. Phylogenetic analysis shows that the strain MBLA0099 T belongs to the genus Halorubrum . The average nucleotide and amino acid identity, and in silico DNA-DNA hybridization values were below the species delineation threshold. Pan-genomic analysis revealed that 3.2% of all genes present in strain MBLA0099 T were unique to the strain. The red carotenoid produced by strain MBLA0099 T was subjected to spectrometric and chromatographic analyses and confirmed to be bacterioruberin as C50 carotenoid. Mevalonic acid, terpenoid backbone, and carotenoid biosynthesis pathway were annotated for strain MBLA0099 T . The C50 carotenoid production by strain MBLA0099 T was also enhanced under various stress conditions including relatively netural pH, high oxidative and salinity conditions. Additionally, the strain MBLA0099 T -derived bacterioruberin showed the antioxidant activity with EC 50 value of 12.29 µg/ml, based on the evaluation of DPPH free radical scavenging activity. The present study would be the first report on the identification of C 50 carotenoid from the strain MBLA0099 T representing a novel species of the genus Halorubrum , for which the name Halorubrum ruber sp. nov. is proposed. The typestrain used was MBLA0099 T (= KCTC 4296 T = JCM 34701 T ).