Details

Autor(en) / Beteiligte

• DORBAN, Gauthier
• DEFAWEUX, Valérie
• DEMONCEAU, Caroline
• FLANDROY, Sylvain
• VAN LERBERGHE, Pierre-Bernard
• FALISSE-POIRRIER, Nandini
• PIRET, Joëlle
• HEINEN, Ernst
• ANTOINE, Nadine

Titel

Interaction between dendritic cells and nerve fibres in lymphoid organs after oral scrapie exposure

Ist Teil von

• Virchows Archiv : an international journal of pathology, 2007-12, Vol.451 (6), p.1057-1065

Ort / Verlag

Berlin: Springer

Erscheinungsjahr

2007

Quelle

SpringerLink

Beschreibungen/Notizen

• In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting the nervous system. To clarify the cellular requirements for the neuroinvasion of the scrapie agent from the lymphoid organs to the central nervous system, we have studied, by confocal microscopy, the innervations within Peyer's patches, mesenteric lymph nodes and the spleen of mice in physiological conditions and after oral exposure to prion. Contacts between nerve fibres and PrPsc-associated cells, dendritic cells (DCs) and follicular dendritic cells (FDCs), were evaluated in preclinical prion-infected mice. Using a double immunolabelling strategy, we demonstrated the lack of innervation of PrPsc-accumulating cells (FDCs). Contacts between nerve fibers and PrPsc-propagating cells (DCs) were detected in T-cell zones and cell-trafficking areas. This supports, for the first time, the possible implication of dendritic cells in the prion neuroinvasion process.