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BibTeX
Expression of the green fluorescent protein in the oligodendrocyte lineage: a transgenic mouse for developmental and physiological studies
Journal of neuroscience research, 2002-11, Vol.70 (4), p.529-545
Yuan, Xiaoqing
Chittajallu, Ramesh
Belachew, Shibeshih
Anderson, Stacie
McBain, Chris J
Gallo, Vittorio
2002
Details
Autor(en) / Beteiligte
Yuan, Xiaoqing
Chittajallu, Ramesh
Belachew, Shibeshih
Anderson, Stacie
McBain, Chris J
Gallo, Vittorio
Titel
Expression of the green fluorescent protein in the oligodendrocyte lineage: a transgenic mouse for developmental and physiological studies
Ist Teil von
Journal of neuroscience research, 2002-11, Vol.70 (4), p.529-545
Ort / Verlag
New York: Wiley Interscience
Erscheinungsjahr
2002
Link zum Volltext
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
We generated a transgenic mouse expressing the enhanced green fluorescent protein (EGFP) under the control of the 2′‐3′‐cyclic nucleotide 3′‐phosphodiesterase (CNP) promoter. EGFP+ cells were visualized in live tissue throughout embryonic and postnatal development. Immunohistochemical analysis in brain tissue and in sciatic nerve demonstrated that EGFP expression was restricted to cells of the oligodendrocyte and Schwann cell lineages. EGFP was also strongly expressed in “adult” oligodendrocyte progenitors (OPs) and in gray matter oligodendrocytes. Fluorescence‐activated cell sorting allowed high‐yield purification of EGFP+ oligodendrocyte‐lineage cells from transgenic brains. Electrophysiological patch clamp recordings of EGFP+ cells in situ demonstrated that OP cells displayed large outward tetraethylammonium (TEA)‐sensitive K+ currents and very small inward currents, whereas mature oligodendrocytes were characterized by expression of large inward currents and small outward K+ currents. The proliferation rate of EGFP+ cells in developing white matter decreased with the age of the animals and was strongly inhibited by TEA. Oligodendrocyte development and physiology can be studied in live tissue of CNP‐EGFP transgenic mice, which represent a source of pure EGFP+ oligodendrocyte‐lineage cells throughout development. © 2002 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0360-4012, 1097-4547
eISSN: 1097-4547
DOI: 10.1002/jnr.10368
Titel-ID: cdi_liege_orbi_v2_oai_orbi_ulg_ac_be_2268_5030
Format
–
Schlagworte
2',3'-Cyclic-Nucleotide Phosphodiesterases - genetics
,
2′-3′-cyclic nucleotide 3′-phosphodiesterase promoter
,
Animals
,
Biochemistry
,
Biochemistry, biophysics & molecular biology
,
Biochimie, biophysique & biologie moléculaire
,
biophysics
,
Brain - cytology
,
Brain - embryology
,
Brain - metabolism
,
Brain/cytology/embryology/metabolism
,
Cell Division - drug effects
,
Cell Lineage - physiology
,
Cells, Cultured
,
Flow Cytometry
,
glia
,
Green Fluorescent Proteins
,
Immunohistochemistry
,
In Vitro Techniques
,
Life sciences
,
Luminescent Proteins - biosynthesis
,
Luminescent Proteins - genetics
,
Luminescent Proteins/biosynthesis/genetics
,
Mice
,
Mice, Transgenic
,
Models, Animal
,
molecular biology
,
Oligodendroglia - cytology
,
Oligodendroglia - drug effects
,
Oligodendroglia - metabolism
,
Oligodendroglia/cytology/drug effects/metabolism
,
Patch-Clamp Techniques
,
Peripheral Nervous System - cytology
,
Peripheral Nervous System - metabolism
,
Peripheral Nervous System/cytology/metabolism
,
Potassium Channel Blockers - pharmacology
,
potassium channels
,
Potassium Channels - drug effects
,
Potassium Channels - metabolism
,
Potassium Channels/drug effects/metabolism
,
Promoter Regions, Genetic
,
Schwann cells
,
Schwann Cells - cytology
,
Schwann Cells - metabolism
,
Schwann Cells/cytology/metabolism
,
Sciences du vivant
,
Stem Cells - cytology
,
Stem Cells - drug effects
,
Stem Cells - metabolism
,
Stem Cells/cytology/drug effects/metabolism
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