Details

Autor(en) / Beteiligte

• Masujin, Kentaro
• Shu, Yujing
• Yamakawa, Yoshio
• Hagiwara, Ken'ichi
• Sata, Tetsutaro
• Matsuura, Yuichi
• Iwamaru, Yoshifumi
• Imamura, Morikazu
• Okada, Hiroyuki
• Mohri, Shirou
• Yokoyama, Takashi

Titel

Biological and biochemical characterization of L-type-like bovine spongiform encephalopathy (BSE) detected in Japanese black beef cattle

Ist Teil von

• Prion, 2008-07, Vol.2 (3), p.123-128

Ort / Verlag

United States: Taylor & Francis

Erscheinungsjahr

2008

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• A case of L-type-like atypical bovine spongiform encephalopathy was detected in 14-year-old Japanese black beef cattle (BSE/JP24).  To clarify the biological and biochemical properties of the prion in BSE/JP24, we performed a transmission study with wild-type mice and bovinized transgenic mice (TgBoPrP).  The BSE/JP24 prion was transmitted to TgBoPrP mice with the incubation period of 199.7 ± 3.4 days, which was shorter than that of classical BSE (C-BSE) (223.5 ± 13.5 days).  Further, C-BSE was transmitted to wild-type mice with the incubation period of about 409 days, whereas BSE/JP24 prion inoculated mice showed no clinical signs up to 649 days.  Severe vacuolation and a widespread and uniform distribution of PrPSc were pathologically observed in the brain of BSE/JP24 prion affected TgBoPrP mice.  The molecular weight and glycoform ratio of PrPSc in BSE/JP24 were different from those in C-BSE, and PrPSc in BSE/JP24 exhibited weaker proteinase K resistance than that in C-BSE.  These findings revealed that the BSE/JP24 prion has distinct biological and biochemical properties reported for that of C-BSE.  Interestingly, a shorter incubation period was observed at the subsequent passage of the BSE/JP24 prion to TgBoPrP mice (152.2 ± 3.1 days).  This result implies that BSE/JP24 prion has newly emerged and showed the possibility that L-type BSE prion might be classified into multiple strains.