Details

Autor(en) / Beteiligte

• Soohyun Um
• Hyeongju Jeong
• Joon Soo An
• Se Jin Jo
• Young Ran Kim
• Dong-chan Oh
• Kyuho Moon

Titel

Chromatographic Determination of the Absolute Configuration in Sanjoinine A That Increases Nitric Oxide Production

Ist Teil von

• Biomolecules & therapeutics, 2023-09, Vol.31 (5), p.566-572

Ort / Verlag

한국응용약물학회

Erscheinungsjahr

2023

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• A chiral derivatization strategy with phenylglycine methyl ester (PGME) was employed to develop a straightforward method to determine the absolute configurations of N,N-dimethyl amino acids. The PGME derivatives were analyzed using liquid chromatography- mass spectrometry to identify the absolute configurations of various N,N-dimethyl amino acids based on their elution time and order. The established method was applied to assign the absolute configuration of the N,N-dimethyl phenylalanine in sanjoinine A (4), a cyclopeptide alkaloid isolated from Zizyphi Spinosi Semen widely used as herbal medicine for insomnia. Sanjoinine A displayed production of nitric oxide (NO) in LPS-activated RAW 264.7 cells.