Details

Autor(en) / Beteiligte

• Park, Chaeri
• Song, Yun-Kyoung
• Kim, Young-Hyun
• Jung, Yena
• Park, Young-Ho
• Song, Bong-Seok
• Eom, Taekil
• Kim, Ju-Sung
• Kim, Sang-Hyun
• Kim, Ji-Su
• Kim, Sun-Uk
• Lee, Sang-Rae
• Kim, Ekyune

Titel

Development of a New Type of Recombinant Hyaluronidase Using a Hexahistidine; Possibilities and Challenges in Commercialization

Ist Teil von

• Journal of microbiology and biotechnology, 2019-08, Vol.29 (8), p.1310-1315

Ort / Verlag

한국미생물생명공학회

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and assess its function. Therefore, in order to find the most efficient active hyaluronidase, we produced several shortened hyaluronidases with partial removal of the N- or C-terminal regions. Moreover, we created an enzyme that connected six histidines onto the end of the hyaluronidase C-terminus. This simplified subsequent purification using Ni 2+ affinity chromatography, making it feasible to industrialize this highly active recombinant hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme. Therefore, this simple and effective isolation method could increase the availability of recombinant hyaluronidase for research and clinical purposes.