Details

Autor(en) / Beteiligte

• Dai, Wenwen
• Wu, Yu
• Bi, Jinpeng
• Wang, Jingyu
• Wang, Shuai
• Kong, Wei
• Barbier, Julien
• Cintrat, Jean-Christophe
• Gao, Feng
• Jiang, Zhengran
• Gillet, Daniel
• Su, Weiheng
• Jiang, Chunlai

Titel

Antiviral Effect of Retro-2.1 against Herpes Simplex Virus Type 2 In Vitro

Ist Teil von

• Journal of microbiology and biotechnology, 2018-06, Vol.28 (6), p.849-859

Ort / Verlag

한국미생물생명공학회

Erscheinungsjahr

2018

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Herpes simplex virus type 2 (HSV-2) infection has been a public health concern worldwide. It is the leading cause of genital herpes and a contributing factor to cervical cancer and human immunodeficiency virus (HIV) infection. No vaccine is available yet for the treatment of HSV-2 infection, and routinely used synthetic nucleoside analogs have led to the emergence of drug resistance. The small molecule Retro-2 cycl has been reported to be active against several pathogens by acting on intracellular vesicle transport, which also participates in the HSV-2 lifecycle. Here, we showed that Retro-2.1, which is an optimized, more potent derivative of Retro-2 cycl , could inhibit HSV-2 infection, with 50% inhibitory concentrations of 5.58 μM and 6.35 μM in cytopathic effect inhibition and plaque reduction assays, respectively. The cytotoxicity of Retro-2.1 was relatively low, with a 50% cytotoxicity concentration of 116.5 μM. We also preliminarily identified that Retro-2.1 exerted the antiviral effect against HSV-2 by a dual mechanism of action on virus entry and late stages of infection. Therefore, our study for the first time demonstrated Retro-2.1 as an effective antiviral agent against HSV-2 in vitro with targets distinct from those of nucleoside analogs.