BMB reports, 2017-10, Vol.50 (10), p.516-521
2017
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Details
- Autor(en) / Beteiligte
- Titel
- Airborne particulate matter increases MUC5AC expression by downregulating Claudin-1 expression in human airway cells
- Ist Teil von
- BMB reports, 2017-10, Vol.50 (10), p.516-521
- Ort / Verlag
- 생화학분자생물학회
- Erscheinungsjahr
- 2017
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- CLB 2.0 , a constituent of PM, induces secretion of multiple cytokines and chemokines that regulate airway inflammation. Specifically, IL-6 upregulates CLB 2.0 -induced MUC5AC and MUC1 expression. Interestingly, of the tight junction proteins examined, claudin-1 expression was inhibited by CLB 2.0 . While the overexpression of claudin-1 decreased CLB 2.0 -induced MUC5AC expression, it increased the expression of the anti-inflammatory mucin, MUC1. CLB 2.0 -induced IL-6 secretion was mediated by ROS. The ROS scavenger N-acetylcysteine inhibited CLB 2.0 -induced IL-6 secretion, thereby decreasing the CLB 2.0 -induced MUC5AC expression, whereas CLB 2.0 -induced MUC1 expression increased. CLB 2.0 activated the ERK1/2 MAPK via a ROS-dependent pathway. ERK1/2 downregulated the claudin-1 and MUC1 expressions, whereas it dramatically increased CLB 2.0 -induced MUC5AC expression. These findings suggest that CLB 2.0 -induced ERK1/2 activation acts as a switch for regulating inflammatory conditions though a ROS-dependent pathway. Our data also suggest that secreted IL-6 regulates CLB 2.0 -induced MUC5AC and MUC1 expression via ROS-mediated downregulation of claudin-1 expression to maintain mucus homeostasis in the airway. [BMB Reports 2017; 50(10): 516-521]