Details

Autor(en) / Beteiligte

• Hou, Dian-Yun
• Xu, Hong
• Du, Guang-Yuan
• Lin, Jun-Tang
• Duan, Min
• Guo, Ai-Guang

Titel

Proteome analysis of chloroplast proteins in stage albinism Line of winter wheat (triticum aestivum) FA85

Ist Teil von

• BMB reports, 2009-07, Vol.42 (7), p.450-455

Ort / Verlag

생화학분자생물학회

Erscheinungsjahr

2009

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The "stage albinism line of winter wheat" FA85 was a specific natural mutant strain on leaf color. This physiological mutation was controlled by cytogene. In order to reveal the genetic and biochemical mechanism of albinism, 2-DE was used to investigate the difference of chloroplast protein expression pattern between FA85 and its parent wheat Aibian 1. From the results of 2-DE gels analysis, approximately 683 spots were detected on each gel, and 57 spots were expressed differently at least two-fold. Using MALDI-TOF/TOF MS, 14 of 57 spots were identified, which could be categorized into four classes: carbon metabolism, energy metabolism, defense/stress response and signal transduction. Compared with the parent wheat, the expression of ATPase-$\gamma$ and GP1-$\alpha$ was up-regulated in FA85, and of other proteins was down-regulated. Together, we concluded that the expression of chloroplast proteins had changed obviously in FA85, which might be related to the leaf color mutant.