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In order to characterize the chloroplasts of Korean ginseng as a semi-shade plant and radish as a sun plant, effects of growth light intensity on photosynthetic electron transport (PS) activity in chloroplasts and superoxide (O_2·^-) production in thylakoid membrane by irradiation were investigated. High-light chloroplasts of both plants showed higher PS activities than those grown under low growth light intensity. High PSⅡ and low PSⅠ activities in ginseng chloroplasts (ratio of PSⅡ/PSⅠ:1.1) were observed, but radish chloroplasts showed low PSⅡ and high PSⅠ activities (ratio of PSⅡ/PSⅠ:0.3). PSⅡ activity of both plants was little affected by temperature in ranges of 15-35℃. Activities of whole-chain (PSⅡ+Ⅰ) in ginseng and PSⅠ in radish were increased at high temperature (40℃). Preincubation of chloroplasts at 40℃ during 30 min, as a mild heat stress, caused rapid decrease in PSⅡ and PSⅡ+Ⅰ activities of both plants. However PSⅠ activity was not decreased in ginseng and rather increased in radish. O_2·^- production (NBT reduction) in Mehler reaction in the thylakoid membrane was inhibited by DCMU in both plants. DMBIB inhibited O_2·^- production in ginseng, but radish was insensitive to DMBIB. Electron flow system in ginseng thylakoid membrane was more susceptible to damage of photooxidation than that of radish.
Sprache
Koreanisch
Identifikatoren
ISSN: 1226-9239
eISSN: 1867-0725
Titel-ID: cdi_kiss_primary_242740
Format
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