Details

Autor(en) / Beteiligte

• Gross, Carol
• Hoffman, John
• Ward, Cathy
• Hager, Dayle
• Burdick, Greg
• Berger, Hillard
• Burgess, Richard

Titel

Mutation Affecting Thermostability of Sigma Subunit of Escherichia coli RNA Polymerase Lies near the dnaG Locus at about 66 Min on the E. coli Genetic Map

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1978-01, Vol.75 (1), p.427-431

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1978

Quelle

MEDLINE

Beschreibungen/Notizen

• The Escherichia coli strain, ts-rnp5, originally described in 1975 by G. D. Burdick and H. Berger, is shown to possess an RNA polymerase (RNA nucleotidyltransferase) σ subunit with an activity 4-6 times less thermostable at 45 degrees than σ from wild-type strains. This defect remains associated with the σ polypeptide through a variety of purification stages, including renaturation of σ after its elution from sodium dodecyl sulfate/polyacrylamide gels. The mutation responsible for decreased thermostability of σ , called rpoD1, cotransduces with dnaG and therefore is located at about 66 min on the E. coli genetic map.