Details

Autor(en) / Beteiligte

• Dupin, Nicolas
• Fisher, Cyril
• Kellam, Paul
• Ariad, Sam
• Tulliez, Micheline
• Franck, Nathalie
• Van Marck, Eric
• Salmon, Dominique
• Gorin, Isabelle
• Escande, Jean-Paul
• Weiss, Robin A.
• Alitalo, Kari
• Boshoff, Chris

Titel

Distribution of Human Herpesvirus-8 Latently Infected Cells in Kaposi's Sarcoma, Multicentric Castleman's Disease, and Primary Effusion Lymphoma

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1999-04, Vol.96 (8), p.4546-4551

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• Human herpesvirus 8 (HHV-8, also called KSHV) is linked to the etiopathogenesis of Kaposi's sarcoma (KS), multicentric Castleman's disease (MCD), and primary effusion lymphoma (PEL). The universal presence of HHV-8 in early KS has not yet been shown. We used a mAb (LN53) against latent nuclear antigen-1 (LNA-1) of HHV-8 encoded by ORF73 to study the distribution of the cell types latently infected by HHV-8 in patch, plaque, and nodular KS, MCD, and PEL. In early KS, HHV-8 is present in <10% of cells forming the walls of ectatic vessels. In nodular KS, HHV-8 is present in cells surrounding slit-like vessels and in >90% of spindle cells, but not in normal vascular endothelium. In addition, HHV-8 colocalizes with vascular endothelial growth factor receptor-3 (VEGFR-3), a marker of lymphatic and precursor endothelium. In early KS lesions, VEGFR-3 is more extensively expressed than LNA-1, indicating that HHV-8 is not inducing the proliferation of VEGFR-3-positive endothelium directly. In MCD, HHV-8 is present in mantle zone large immunoblastic B cells. No staining for LNA-1 is seen in samples from multiple myeloma, prostate cancer, and angiosarcoma, supporting the absence of any etiological link between these diseases and HHV-8.