Proceedings of the National Academy of Sciences - PNAS, 1993-04, Vol.90 (8), p.3452-3456
1993
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Two Members of a Distinct Subfamily of 5-Hydroxytryptamine Receptors Differentially Expressed in Rat Brain
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1993-04, Vol.90 (8), p.3452-3456
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1993
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We report two serotonin (5-hydroxytryptamine, 5-HT) receptors, MR22 and REC17, that belong to the G-protein-associated receptor superfamily. MR22 and REC17 are 371 and 357 amino acids long, respectively, as deduced from nucleotide sequence and share 68% mutual amino acid identity and 30-35% identity with known catecholamine and 5-HT receptors. Saturable binding of125I-labeled (+)-lysergic acid diethylamide to transiently expressed MR22 in COS-M6 cells was inhibited by ergotamine > methiothepin > 5-carboxamidotryptamine > 5-HT. For REC17, the rank of potency was ergotamine > 5-carboxamidotryptamine > methiothepin > methysergide > 5-HT. Both were insensitive to 5-HT1A, 5-HT1Dor 5-HT2serotonergic ligands [8-hydroxy-2-(di-n-propylamino)tetralin, sumatriptan, and 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane]. The mRNAs encoding MR22 were detected in the CA1 region of hippocampus, the medial habenula, and raphe nuclei. In contrast, mRNAs encoding REC17 were found throughout the rat central nervous system. We propose that REC17 and MR22, designated as 5-HT5αand 5-HT5β, represent a distinct subfamily of 5-HT receptors.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.90.8.3452Titel-ID: cdi_jstor_primary_2361762
- Format
- –
- Schlagworte
- 5-hydroxytryptamine, Amino Acid Sequence, Amino acids, Animals, Base Sequence, binding, Biological and medical sciences, brain, Brain - physiology, Cell Line, Cell receptors, Cell structures and functions, Cellular biology, Cloning, Molecular - methods, Complementary DNA, detection, Fundamental and applied biological sciences. Psychology, GTP-Binding Proteins - genetics, HeLa Cells, Hippocampus, Humans, Hypothalamus - physiology, Identity, Ligands, Messenger RNA, Molecular and cellular biology, Molecular Sequence Data, Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine), mRNA, Multigene Family, Nervous system, Neurology, Oligodeoxyribonucleotides, Poly A - genetics, Poly A - isolation & purification, Polymerase chain reaction, Polymerase Chain Reaction - methods, properties, Rats, Receptors, Receptors, Serotonin - biosynthesis, Receptors, Serotonin - genetics, Receptors, Serotonin - metabolism, RNA, RNA - genetics, RNA - isolation & purification, RNA, Messenger - genetics, RNA, Messenger - metabolism, Serotonin receptors, serotonin S5 alpha, serotonin S5 beta, Transfection