Details

Autor(en) / Beteiligte

• IKEGAYA, Kazuko
• TSUCHIYA, Ken
• SARATANI, Kazuma

Titel

Clinical Significance of Bacterial Identification by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) Combined with Detection of Drug Resistance Genes in Blood Culture

Ist Teil von

• Kansenshogaku Zasshi, 2022/07/20, Vol.96(4), pp.118-124

Ort / Verlag

The Japanese Association for Infectious Diseases

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Direct identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in blood culture combined with detection of drug resistance genes may improve patient outcomes based on prompt identification of pathogenic microorganisms and prediction of drug resistance. In this study, the clinical effects of bacterial identification by MALDI-TOF-MS and detection of drug resistance genes were evaluated.Time from starting the test to obtaining a report identifying bacteria, time until appropriate escalation, time until de-escalation, and mortality within 30 days were compared from before to after the intervention; i. e., before and after use of mass spectrometry evaluation and a drug resistance gene test.A total of 159 specimens were examined before the intervention and 197 after the intervention. Direct identification by mass spectrometry and detection of drug resistance genes decreased the time to identify the bacteria (34.05 vs.4.58 hours, p< 0.0001), time until appropriate escalation (37.27 vs.9.47 hours, p< 0.0001), time until de-escalation (75.98 vs.57.48 hours, p=0.0029), and the 30-day mortality (10.1% vs.4.1%, p=0.026).These results suggest that prompt identification by mass spectrometry combined with detection of drug resistance genes may improve the prognosis of patients.