Details

Autor(en) / Beteiligte

• OZUDOGRU, E. A
• KIRDOK, E
• KAYA, E
• CAPUANA, M
• BENELLI, C
• ENGELMANN, F

Titel

Cryopreservation of Redwood (Sequoia Sempervirens (D. DON.) ENDL.) in Vitro Buds Using Vitrification-Based Techniques

Ist Teil von

• Cryo-Letters, 2011-03, Vol.32 (2), p.99-110

Ort / Verlag

Lewes: Cryoletters

Erscheinungsjahr

2011

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation-vitrification and droplet-vitrification were compared for cryopreserving Sequoia sempervirens apical and basal buds sampled from in vitro shoot cultures. The effect of cold-hardening of mother-plants and of bud culture medium and sucrose preculture was also investigated. Culture of apical and basal buds sampled from coldhardened mother-plants on Quoirin and Lepoivre medium with activated charcoal had a positive effect on regrowth. Only droplet-vitrification ensured survival and regrowth after cryopreservation. After cryopreservation, regeneration of apical buds was possible for PVS2 exposure durations between 90 and 180 min but it remained low, with a maximum of 18% after 135 min treatment. With basal buds, regeneration after cryopreservation was possible over a larger range of PVS2 treatment durations, between 30 and 180 min. The highest regeneration percentage was slightly higher (22%) than that measured with apical buds, and was also achieved after 135 min PVS2 exposure.