Details
- Autor(en) / Beteiligte
- Titel
- miR-200b targets GATA-4 during cell growth and differentiation
- Ist Teil von
- RNA biology, 2013-04, Vol.10 (4), p.465-480
- Ort / Verlag
- United States: Taylor & Francis
- Erscheinungsjahr
- 2013
- Link zum Volltext
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- GATA-4 is an important transcription factor involved in several developmental processes of the heart, such as cardiac myocyte proliferation, differentiation and survival. The precise mechanisms underlying the regulation of GATA-4 remain unclear, this is especially true for the mechanisms that mediate the post-transcriptional regulation of GATA-4. Here, we demonstrate that miR-200b, a member of the miR-200 family, is a critical regulator of GATA-4. Overexpression of miR-200b leads to the downregulation of GATA-4 mRNA and a decrease in GATA-4 protein levels. Moreover, miR-200b not only inhibits cell growth and differentiation but also reverses the growth response mediated by GATA-4, whereas depletion of miR-200b leads to a slight reversal of the anti-growth response achieved by knocking down endogenous GATA-4. More importantly, the cell cycle-associated gene cyclin D1, which is a downstream target of GATA-4, is also regulated by miR-200b. Thus, miR-200b targets GATA-4 to downregulate the expression of cyclin D1 and myosin heavy chain (MHC), thereby regulating cell growth and differentiation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1547-6286eISSN: 1555-8584DOI: 10.4161/rna.24370Titel-ID: cdi_informaworld_taylorfrancis_310_4161_rna_24370
- Format
- –
- Schlagworte
- Animals, Apoptosis - genetics, Cell Cycle - genetics, Cell Cycle - physiology, Cell Cycle Checkpoints - genetics, cell differentiation, Cell Differentiation - genetics, Cell Differentiation - physiology, cell growth, Cell Line, Cell Line, Tumor, Cell Proliferation, Cyclin D1 - genetics, Cyclin D1 - metabolism, GATA-4, GATA4 Transcription Factor - genetics, GATA4 Transcription Factor - metabolism, Gene Expression Regulation, Humans, Mice, MicroRNAs - genetics, MicroRNAs - metabolism, miR-200b, Muscle Development - genetics, Myocytes, Cardiac - metabolism, Myosin Heavy Chains - genetics, Myosin Heavy Chains - metabolism, RNA Families, transcription regulation