Nanotoxicology, 2019-02, Vol.13 (2), p.174-188
2019
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- Autor(en) / Beteiligte
- Titel
- Identification of signaling cascade in the insulin signaling pathway in response to nanopolystyrene particles
- Ist Teil von
- Nanotoxicology, 2019-02, Vol.13 (2), p.174-188
- Ort / Verlag
- England: Taylor & Francis
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The molecular response of animals to nanoplastic particles is still largely unclear. In this study, we employed a modified prolonged exposure system to investigate the molecular response of Caenorhabditis elegans to nanopolystyrene particles. Exposure to nanopolystyrene particles (1 μg/L) significantly decreased expressions of daf-2 encoding an insulin receptor, age-1 encoding a PI3K, and akt-1 encoding an Akt/PKB, and increased expression of daf-16 encoding a FOXO transcriptional factor in insulin signaling pathway. Among these genes, mutation of daf-2, age-1, or akt-1 induced a resistance to toxicity of nanopolystyrene particles, whereas mutation of daf-16 induced a susceptibility to the toxicity of nanopolystyrene particles. RNAi knockdown of daf-16 could further suppress the resistance of daf-2, age-1, or akt-1 mutant to the toxicity of nanopolystyrene particles. The insulin signaling pathway acted in intestinal cells to regulate the toxicity of nanopolystyrene particles. Moreover, sod-3 encoding a manganese superoxide dismutase, mtl-1 encoding a metallothionein, and gpd-2 encoding a glyceraldehyde-3-phosphate dehydrogenase were identified as downstream targeted genes for daf-16 in the regulation of toxicity of nanopolystyrene particles. Therefore, a signaling cascade of DAF-2-AGE-1-AKT-1-DAF-16-SOD-3/MTL-1/GPD-2 was identified in response to nanopolystyrene particles in nematodes. Additionally, this signaling cascade in the insulin signaling pathway may mediate a protective response for nematodes against the adverse effects from nanopolystyrene particles.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1743-5390eISSN: 1743-5404DOI: 10.1080/17435390.2018.1530395Titel-ID: cdi_informaworld_taylorfrancis_310_1080_17435390_2018_1530395
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, Age, AKT protein, AKT1 protein, Animals, Caenorhabditis elegans, Caenorhabditis elegans - drug effects, Caenorhabditis elegans - genetics, Caenorhabditis elegans - metabolism, Caenorhabditis elegans Proteins - genetics, Coding, Dose-Response Relationship, Drug, Exposure, Forkhead protein, Gene expression, Genes, Glucose, Glyceraldehyde-3-phosphate dehydrogenase, Insulin, Insulin - genetics, Insulin - metabolism, insulin signaling pathway, Intestine, Intestines - drug effects, Manganese, Metallothionein, Mutation, Nanoparticles - chemistry, Nanoparticles - toxicity, Nanopolystyrene, Nematodes, Particulates, Phosphatidylinositol 3-Kinases - genetics, Polystyrenes - chemistry, Polystyrenes - toxicity, Proto-Oncogene Proteins c-akt - genetics, Receptor, Insulin - genetics, response, RNA-mediated interference, Signal Transduction, Signaling, Superoxide dismutase, Toxicity