Details

Autor(en) / Beteiligte

• Petreni, Andrea
• De Luca, Viviana
• Scaloni, Andrea
• Nocentini, Alessio
• Capasso, Clemente
• Supuran, Claudiu T.

Titel

Anion inhibition studies of the Zn(II)-bound ι-carbonic anhydrase from the Gram-negative bacterium Burkholderia territorii

Ist Teil von

• Journal of enzyme inhibition and medicinal chemistry, 2021-01, Vol.36 (1), p.372-376

Ort / Verlag

England: Taylor & Francis

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Taylor & Francis Journals Auto-Holdings Collection

Beschreibungen/Notizen

• Burkholderia territorii, a Gram-negative bacterium, encodes for the ι-class carbonic anhydrase (CA, EC 4.2.1.1) BteCAι, which was recently characterised. It acts as a good catalyst for the hydration of CO 2 to bicarbonate and protons, with a k cat value of 3.0 × 10 5  s −1 and k cat /K M value of 3.9 × 10 7  M −1 s −1 . No inhibition data on this new class of enzymes are available to date. We report here an anion and small molecules inhibition study of BteCAι, which we prove to be a zinc(II)- and not manganese(II)-containing enzyme, as reported for diatom ι-CAs. The best inhibitors were sulphamic acid, stannate, phenylarsonic acid, phenylboronic acid and sulfamide (K I values of 6.2-94 µM), whereas diethyldithiocarbamate, tellurate, selenate, bicarbonate and cyanate were submillimolar inhibitors (K I values of 0.71-0.94 mM). The halides (except iodide), thiocyanate, nitrite, nitrate, carbonate, bisulphite, sulphate, hydrogensulfide, peroxydisulfate, selenocyanate, fluorosulfonate and trithiocarbonate showed K I values in the range of 3.1-9.3 mM.