Details

Autor(en) / Beteiligte

• Bourassa, P.
• Chanphai, P.
• Tajmir-Riahi, H.A.

Titel

Folic acid delivery by serum proteins: loading efficacy and protein morphology

Ist Teil von

• Journal of biomolecular structure & dynamics, 2017-12, Vol.35 (16), p.3499-3506

Ort / Verlag

England: Taylor & Francis

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Taylor & Francis

Beschreibungen/Notizen

• The loading efficacy of folic acid with serum proteins human serum albumin (HSA), bovine serum albumin (BSA), and beta-lactoglobulin (β-LG) was analyzed and the effect of acid conjugation on protein morphology was determined. Structural analysis showed that folic acid binds HSA, BSA, and β-LG via hydrophilic, hydrophobic, and H-bonding contacts with BSA forming more stable conjugates than HSA and β-LG. Molecular modeling showed the presence of several H-bonding systems, stabilizing acid-protein conjugates. Folic acid conjugation alters protein conformation by major alterations of α-helix and β-sheet. TEM images showed major protein morphological changes inducing protein aggregation upon acid interaction. The results show that serum proteins can deliver folic acid to target molecules.