Details

Autor(en) / Beteiligte

• Sandhu, Noreen
• Karlsen, Mona A.
• Høgdall, Claus
• Laursen, Inga A.
• Christensen, Ib J.
• Høgdall, Estrid V. S.

Titel

Stability of HE4 and CA125 in blood samples from patients diagnosed with ovarian cancer

Ist Teil von

• Scandinavian journal of clinical and laboratory investigation, 2014-09, Vol.74 (6), p.477-484

Ort / Verlag

England: Informa Healthcare

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract Objective. To investigate the influence of handling and storage on HE4 and CA125 serum and EDTA plasma levels to clarify any important consequences for a clinical setting. Methods. Blood samples from 13 ovarian cancer (OC) patients were collected and allowed to clot or sediment for up to 72 hours at 4°C or 20°C, then processed into serum and EDTA plasma. Furthermore, the effects of up to eight repetitive cycles of freeze/thaw were investigated. HE4 and CA125 were analyzed using a Chemiluminescent Microparticle Immunoassay on the Architect i2000sr System. Results. No significant effect of processing time for HE4 could be shown. HE4 EDTA plasma levels were insignificantly lower (3%) than serum levels (p = 0.41). Similarly, no significant effect of processing time for CA125 could be demonstrated. CA125 levels at 4°C were significantly reduced compared to levels at 20°C (p = 0.024). No significant difference between CA125 serum and plasma levels were found (p = 0.46). Serum and EDTA plasma samples were stable during the eight cycles of freezing and thawing (CA125: all p > 0.2; HE4: all p > 0.5). Conclusion. No systematic difference could be demonstrated for HE4. CA125 is not dependent on processing time, EDTA plasma or serum. Levels of CA125 are significantly reduced at 4°C compared to levels at 20°C, but this difference was less than 6% and is not considered clinically relevant.