Details

Autor(en) / Beteiligte

• Ye, Zhi Ting
• Tseng, Shen Fu
• Tsou, Shang Xuan
• Kuo, Hsin Ching
• Ke, Chi Wu
• Yen, Kuang Chen

Titel

A Lightweight Paper Sensor for Real-Time Detection of Human Serum Albumin Based on Imaging and Spectroscopy Methods

Ist Teil von

• IEEE sensors journal, 2024-06, Vol.24 (11), p.17630-17637

Ort / Verlag

IEEE

Erscheinungsjahr

2024

Quelle

IEEE Xplore

Beschreibungen/Notizen

• Detecting albumin levels quantitatively is challenging due to interference from other substances and lengthy reaction times. In this research, we proposed a lightweight paper sensor for real-time human serum albumin (HSA) detection, utilizing imaging and spectroscopy techniques. By combining bromocresol green (BCG) with HSA, we induced a structural change in BCG, resulting in protonation from <inline-formula> <tex-math notation="LaTeX">\text{C}_{{21}}\text{H}_{{14}} </tex-math></inline-formula>Br4O5S to <inline-formula> <tex-math notation="LaTeX">\text{C}_{{21}}\text{H}_{{12}} \mathrm{Br}_4 \mathrm{O}_5^{2-} \mathrm{S} </tex-math></inline-formula>, leading to a blue-green coloration. Subsequently, we applied this reagent to test paper for the reaction, utilized a smartphone camera for image detection, and performed spectrophotometric analyses. Our study presents a convenient, swift, and quantitative approach to measuring HSA concentration, initially accessible through a smartphone camera. In addition, spectrophotometric analysis ensures precise quantification.