Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Extracellular water measurements: organ tracer kinetics of bromide and sucrose in rats and man
Ist Teil von
American journal of physiology. Renal, fluid and electrolyte physiology, 1978-09, Vol.235 (3), p.254
Ort / Verlag
United States
Erscheinungsjahr
1978
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
R. N. Pierson Jr, D. C. Price, J. Wang and R. K. Jain
Bromide and sucrose distributions were measured as functions of time after
tracer injection into 14 rat organs that accounted for 93% of body wt, with
the goal of evaluating the use of bromide and sucrose as tracers for the
extracellular water volume (ECW). The tracers, Na, 82Br, 125I-labeled human
serum albumin, [14C]sucrose, and 3H2O, were used to calculate bromide and
sucrose content in red cells, plasma, and 13 blood-free organs. Selective
concentration of Br- occurs in RBC, stomach, and skin, accounting in part
for the discrepancy between the Br- space and the smaller ECW volume as
derived from other methods. Sucrose is rapidly metabolized in the rat and
its 14C tracer cannot be used for ECW determination in this species. The
kinetics of Br- distribution were estimated in rats and in 16 human
subjects by measuring plasma disappearance values and specific organ
uptakes. A pharmacokinetic compartmental model was derived, containing
explicit parameters for blood flow, diffusion constants, and ECW spaces
separately for each organ. Precise fitting of experimental bromide data was
achieved for the rat; satisfactory fitting was also achieved in man from
more limited plasma and biopsy data.