Details

Autor(en) / Beteiligte

• Yang, Rong-Ze
• Lee, Mi-Jeong
• Hu, Hong
• Pray, Jessica
• Wu, Hai-Bin
• Hansen, Barbara C
• Shuldiner, Alan R
• Fried, Susan K
• McLenithan, John C
• Gong, Da-Wei

Titel

Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action

Ist Teil von

• American journal of physiology: endocrinology and metabolism, 2006-06, Vol.290 (6), p.E1253-E1261

Ort / Verlag

United States

Erscheinungsjahr

2006

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Division of Endocrinology, Diabetes and Nutrition; 2 Division of Gerontology and 3 Obesity and Diabetes Research Center, University of Maryland School of Medicine at Baltimore; and 4 Geriatrics Research and Education Clinical Center, Veterans Affairs Medical Center, Baltimore, Maryland Submitted 6 December 2004 ; accepted in final form 20 January 2006 Central (visceral) obesity is more closely associated with insulin resistance, type 2 diabetes, and cardiovascular disease than is peripheral [subcutaneous (sc)] obesity, but the underlying mechanism for this pathophysiological difference is largely unknown. To understand the molecular basis of this difference, we sequenced 10,437 expressed sequence tags (ESTs) from a human omental fat cDNA library and discovered a novel visceral fat depot-specific secretory protein, which we have named omentin. Omentin ESTs were more abundant than many known adipose genes, such as perilipin, adiponectin, and leptin in the cDNA library. Protein sequence analysis indicated that omentin mRNA encodes a peptide of 313 amino acids, containing a secretory signal sequence and a fibrinogen-related domain. Northern analysis demonstrated that omentin mRNA was predominantly expressed in visceral adipose tissue and was barely detectable in sc fat depots in humans and rhesus monkeys. Quantative real-time PCR showed that omentin mRNA was expressed in stromal vascular cells, but not fat cells, isolated from omental adipose tissue, with >150-fold less in sc cell fractions. Accordingly, omentin protein was secreted into the culture medium of omental, but not sc, fat explants. Omentin was detectable in human serum by Western blot analysis. Addition of recombinant omentin in vitro did not affect basal but enhanced insulin-stimulated glucose uptake in both sc (47%, n = 9, P = 0.003) and omental ( 30%, n = 3, P < 0.05) human adipocytes. Omentin increased Akt phosphorylation in the absence and presence of insulin. In conclusion, omentin is a new adipokine that is expressed in omental adipose tissue in humans and may regulate insulin action. obesity; diabetes; insulin resistance; visceral fat; metabolic syndrome Address for reprint requests and other correspondence: D. Gong, 660 W. Redwood St., Rm. 497, Baltimore, MD 21201 (e-mail: dgong{at}medicine.umaryland.edu )