American journal of physiology: endocrinology and metabolism, 2003-01, Vol.284 (1), p.E237-E239
2003
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Details
- Autor(en) / Beteiligte
- Titel
- IGF-I and IGFBP-3 transport in the rat heart
- Ist Teil von
- American journal of physiology: endocrinology and metabolism, 2003-01, Vol.284 (1), p.E237-E239
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2003
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- 1 Veterans Administration Medical Center and Departments of 2 Internal Medicine and 3 Anatomy and Cell Biology, 4 Diabetes and Endocrinology Research Center, The University of Iowa, Iowa City, Iowa 52246 Specific binding of IGF-binding protein (IGFBP)-3 was shown to be present in the isolated, beating rat heart. The uptake of perfused 125 I-labeled IGF-I in the beating heart was decreased to 9% by blocking IGF-I binding sites with the IGF-I analog Long R 3 (LR 3 ) IGF-I. When LR 3 was perfused with complexes of 125 I-IGF-I · IGFBP-3, uptake of 125 I-IGF-I was decreased to 41%, which was significantly greater than LR 3 and 125 I-IGF-I (41 vs. 9%). These data suggest that both microvessel IGF-I and IGFBP-3 binding sites contribute to the transport of IGF-I in the perfused rat heart. This also suggests a novel and plausible mechanism whereby circulating IGFs reach sites of IGF bioactivity. perfused heart; endothelial function; insulin-like growth factor I; insulin-like growth factor-binding protein-3
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0193-1849eISSN: 1522-1555DOI: 10.1152/ajpendo.00336.2002Titel-ID: cdi_highwire_physiology_ajpendo_284_1_E237
- Format
- –
- Schlagworte
- Animals, Autoradiography, Binding Sites, Biological Transport, Cells, Cultured, Endothelium, Vascular - metabolism, Insulin-Like Growth Factor Binding Protein 3 - administration & dosage, Insulin-Like Growth Factor Binding Protein 3 - genetics, Insulin-Like Growth Factor Binding Protein 3 - metabolism, Insulin-Like Growth Factor Binding Protein 4 - genetics, Insulin-Like Growth Factor Binding Protein 4 - metabolism, Insulin-Like Growth Factor I - administration & dosage, Insulin-Like Growth Factor I - metabolism, Iodine Radioisotopes, Male, Microcirculation - metabolism, Myocardium - metabolism, Rats, Rats, Sprague-Dawley, Recombinant Fusion Proteins - metabolism