Details

Autor(en) / Beteiligte

• Kim, Hyoung Kyu
• Park, Won Sun
• Kang, Sung Hyun
• Warda, Mohamad
• Kim, Nari
• Ko, Jae-Hong
• Prince, Abd El-bary
• Han, Jin

Titel

Mitochondrial alterations in human gastric carcinoma cell line

Ist Teil von

• American Journal of Physiology: Cell Physiology, 2007-08, Vol.293 (2), p.C761-C771

Ort / Verlag

United States: American Physiological Society

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Mitochondrial Signaling Laboratory, Mitochondria Research Group, Department of Physiology and Biophysics, College of Medicine, Biohealth Products Research Center, Cardiovascular and Metabolic Disease Research Center, Inje University, Busan, Korea; and 2 Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt Submitted 31 January 2007 ; accepted in final form 24 May 2007 We compared mitochondrial function, morphology, and proteome in the rat normal gastric cell line RGM-1 and the human gastric cancer cell line AGS. Total numbers and cross-sectional sizes of mitochondria were smaller in AGS cells. Mitochondria in AGS cells were deformed and consumed less oxygen. Confocal microscopy indicated that the mitochondrial inner membrane potential was hyperpolarized and the mitochondrial Ca 2+ concentration was elevated in AGS cells. Interestingly, two-dimensional electrophoresis proteomics on the mitochondria-enriched fraction revealed high expression of four mitochondrial proteins in AGS cells: ubiquinol-cytochrome c reductase, mitochondrial short-chain enoyl-coenzyme A hydratase-1, heat shock protein 60, and mitochondria elongation factor Tu. The results provide clues as to the mechanism of the mitochondrial changes in cancer at the protein level and may serve as potential cancer biomarkers in mitochondria. two-dimensional gel electrophoresis proteomics; biomarker; cancer Address for reprint requests and other correspondence: J. Han, Mitochondrial Signaling Laboratory, Mitochondria Research Group, Dept. of Physiology and Biophysics, College of Medicine, Biohealth Products Research Center, Cardiovascular and Metabolic Disease Center, Inje Univ. 633-165 Gaegeum-Dong, Busanjin-Gu, Busan 613-735, Korea (e-mail: phyhanj{at}ijnc.inje.ac.kr )