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Electrically silent potassium channel subunits from human lens epithelium
Ist Teil von
American Journal of Physiology: Cell Physiology, 1999-09, Vol.277 (3), p.C412-C424
Ort / Verlag
United States
Erscheinungsjahr
1999
Quelle
MEDLINE
Beschreibungen/Notizen
Departments of Physiology/Biophysics and Ophthalmology, Mayo
Foundation, Rochester, Minnesota 55905
We describe the cloning and characterization of the first human
members, hKv9.1 and hKv9.3, of the electrically silent
delayed-rectifying-like K + channel
subfamily. Their modulatory effects on the electrically active
subfamily member hKv2.1 are also quantified. The hKv9
K + channels were isolated from a
human lens epithelium cDNA library, but both hKv9.1 mRNA and hKv9.3
mRNA were found to coexist with the mRNA for hKv2.1 in a large number
of human tissues. The hKv9.1 gene is composed of a minimum of five
exons, with at least two alternatively spliced exons in the
5'-untranslated region (UTR). In contrast, the hKv9.3 gene is
intronless across the coding region, 3'-UTR, and all of the
analyzed 5'-UTR. Radiation hybrid mapping localized the hKv9.1
gene to 20q12 and the hKv9.3 gene to 2p24. Each electrically silent
subunit, when coexpressed with hKv2.l, slows deactivation and
inactivation compared with hKv2.1 expressed alone. In addition, each
results in an increment in the single channel conductance.
delayed rectifier; hKv9.1; hKv9.3; transfection; Chinese hamster
ovary cells