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Efficient Binding of NC2·TATA-binding Protein to DNA in the Absence of TATA
Ist Teil von
The Journal of biological chemistry, 2005-02, Vol.280 (7), p.6222
Ort / Verlag
American Society for Biochemistry and Molecular Biology
Erscheinungsjahr
2005
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Negative cofactor 2 (NC2) forms a stable complex with TATA-binding protein (TBP) on promoters. This prevents the assembly
of transcription factor (TF) IIA and TFIIB and leads to repression of RNA polymerase II transcription. Here we have revisited
the interactions of NC2·TBP with DNA. We show that NC2·TBP complexes exhibit a significantly reduced preference for TATA box
sequences compared with TBP and TBP·TFIIA complexes. In chromatin immunoprecipitations, NC2 is found on a variety of human
TATA-containing and TATA-less promoters. Substantial amounts of NC2 are present in a complex with TBP in bulk chromatin. A
complex of NC2·TBP displays a K D for DNA of â¼2 à 10 -9 m for a 35-bp major late promoter oligonucleotide. While preferentially recognizing promoter-bound TBP, NC2 also accelerates
TBP binding to promoters and stabilizes TBP·DNA complexes. Our data suggest that NC2 controls TBP binding and maintenance
on DNA that is largely independent of a canonical TATA sequence.
Sprache
Englisch
Identifikatoren
ISSN: 0021-9258
eISSN: 1083-351X
DOI: 10.1074/jbc.M406343200
Titel-ID: cdi_highwire_biochem_280_7_6222
Format
–
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