Details

Autor(en) / Beteiligte

• Faure, G.
• Shelukhina, I. V.
• Porowinska, D.
• Shulepko, M. A.
• Lyukmanova, E. N.
• Dolgikh, D. A.
• Spirova, E. N.
• Kasheverov, I. E.
• Utkin, Yu. N.
• Corringer, J. -P.
• Tsetlin, V. I.

Titel

Interaction of three-finger proteins from snake venoms and from mammalian brain with the cys-loop receptors and their models

Ist Teil von

• Doklady. Biochemistry and biophysics, 2016-05, Vol.468 (1), p.193-196

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• With the use of surface plasmon resonance (SPR) it was shown that ws-Lynx1, a water-soluble analog of the three-finger membrane-bound protein Lynx1, that modulates the activity of brain nicotinic acetylcholine receptors (nAChRs), interacts with the acetylcholine-binding protein (AChBP) with high affinity, K D = 62 nM. This result agrees with the earlier demonstrated competition of ws-Lynx1 with radioiodinated α-bungarotoxin for binding to AChBP. For the first time it was shown that ws-Lynx1 binds to GLIC, prokaryotic Cys-loop receptor ( K D = 1.3 μM). On the contrary, SPR revealed that α-cobratoxin, a three-finger protein from cobra venom, does not bind to GLIC. Obtained results indicate that SPR is a promising method for analysis of topography of ws-Lynx1 binding sites using its mutants and those of AChBP and GLIC.