Details

Autor(en) / Beteiligte

• Guan, Xue Li
• Cestra, Gianluca
• Shui, Guanghou
• Kuhrs, Antje
• Schittenhelm, Ralf B.
• Hafen, Ernst
• van der Goot, F. Gisou
• Robinett, Carmen C.
• Gatti, Maurizio
• Gonzalez-Gaitan, Marcos
• Wenk, Markus R.

Titel

Biochemical Membrane Lipidomics during Drosophila Development

Ist Teil von

• Developmental cell, 2013-01, Vol.24 (1), p.98-111

Ort / Verlag

Cambridge, MA: Elsevier Inc

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Lipids play critical roles in energy homeostasis, membrane structure, and signaling. Using liquid chromatography and mass spectrometry, we provide a comprehensive semiquantification of lipids during the life cycle of Drosophila melanogaster (230 glycerophospholipids, 210 sphingolipids, 6 sterols and sterol esters, and 60 glycerolipids) and obtain biological insights through this biochemical resource. First, we find a high and constant triacylglycerol-to-membrane lipid ratio during pupal stage, which is nonobvious in the absence of nutrient uptake and tissue remodeling. Second, sphingolipids undergo specific changes in headgroup (glycosylation) and tail configurations (unsaturation and hydroxylation on sphingoid base and fatty acyls, respectively), which correlate with gene expression of known (GlcT/CG6437; FA2H/ CG30502) and putative (Cyt-b5-r/CG13279) enzymes. Third, we identify a gender bias in phosphoethanolamine-ceramides as a lead for future investigation into sexual maturation. Finally, we partially characterize ghiberti, required for male meiotic cytokinesis, as a homolog of mammalian serine palmitoyltransferase. ► Resource to derive insights into the role of lipids during Drosophila development ► Hydroxylated sphingolipids undergo extensive changes during Drosophila development ► Phosphoethanolamine ceramides in Drosophila exhibit gender bias ► Male meiotic cytokinesis-required ghiberti gene encodes a serine palmitoyltransferase Guan et al. report a comprehensive semiquantification of lipids through the Drosophila melanogaster life cycle (230 glycerophospholipids, 210 sphingolipids, 6 sterols and sterol esters, 60 glycerolipids), providing insight into lipid changes during development. They further use this resource to implicate the male meiotic cytokinesis gene ghiberti as a serine palmitoyltransferase.