Details
- Autor(en) / Beteiligte
- Titel
- Identification of genes involved in growth inhibition of breast cancer cells transduced with estrogen receptor
- Ist Teil von
- FEBS letters, 2003-10, Vol.553 (3), p.445-450
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2003
- Link zum Volltext
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- Estrogen receptor α (ERα)-negative breast cancer cells display an aggressive phenotype. We previously showed that adenoviral expression of ERα in ER-negative breast cancer cells leads to an estrogen-dependent down-regulation of the proliferation, which could be of interest to control the growth of such cells. In this study, we observed an increase in protein levels of p21 and p27 cyclin-dependent kinase inhibitors, whereas pRb phosphorylation is strongly decreased. Flow cytometry experiments showed a slower transit of cells in G1 (hormone-independent), a hormone-induced accelerated transit through S phase and a possible arrest in G2/M phase. In addition, ERα-expressing cells were undergoing apoptosis. By using cDNA macroarrays, we identified a novel collection of genes regulated by liganded ERα potentially regulating cell cycle, apoptosis, cell signalling, stress response and DNA repair.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(03)01090-1Titel-ID: cdi_hal_primary_oai_HAL_inserm_00143969v1
- Format
- –
- Schlagworte
- Adenoviruses, Human, Adenoviruses, Human - genetics, Adenoviruses, Human - pathogenicity, Apoptosis, Apoptosis - genetics, Breast Neoplasms, Breast Neoplasms - genetics, Breast Neoplasms - metabolism, Breast Neoplasms - pathology, Cancer, cDNA array, Cell Cycle, Cell Cycle - genetics, Cell Division, Cell Division - genetics, Cyclin-Dependent Kinases, Cyclin-Dependent Kinases - antagonists & inhibitors, Down-Regulation, Estradiol, Estradiol - pharmacology, Estrogen, Estrogen Receptor alpha, Flow Cytometry, Gene Expression Regulation, Neoplastic, Gene Expression Regulation, Neoplastic - genetics, Humans, Life Sciences, Microtubule-Associated Proteins, Microtubule-Associated Proteins - antagonists & inhibitors, Oligonucleotide Array Sequence Analysis - methods, Phosphorylation, Proliferation, Receptor, Receptors, Estrogen - biosynthesis, Receptors, Estrogen - genetics, Receptors, Estrogen - metabolism, Recombinant Proteins - genetics, Recombinant Proteins - metabolism, Retinoblastoma Protein - metabolism, Transduction, Genetic, Tumor Cells, Cultured, Up-Regulation