Immunity (Cambridge, Mass.), 2006-02, Vol.24 (2), p.191-201
2006
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- Autor(en) / Beteiligte
- Titel
- Dendritic Cells Rapidly Recruited into Epithelial Tissues via CCR6/CCL20 Are Responsible for CD8 + T Cell Crosspriming In Vivo
- Ist Teil von
- Immunity (Cambridge, Mass.), 2006-02, Vol.24 (2), p.191-201
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The nature of dendritic cell(s) (DC[s]) that conditions efficient in vivo priming of CD8 + CTL after immunization via epithelial tissues remains largely unknown. Here, we show that myeloid DCs rapidly recruited by adjuvants into the buccal mucosa or skin are essential for CD8 + T cell crosspriming. Recruitment of circulating DC precursors, including Gr1 + monocytes, precedes the sequential accumulation of CD11c + MHC class II + DCs in dermis and epithelium via a CCR6/CCL20-dependent mechanism. Remarkably, a defect in CCR6, local neutralization of CCL20, or depletion of monocytes prevents in vivo priming of CD8 + CTL against an innocuous protein antigen administered with adjuvant. In addition, transfer of CCR6-sufficient Gr1 + monocytes restores CD8 + T cell priming in CCR6 °/° mice via a direct Ag presentation mechanism. Thus, newly recruited DCs likely derived from circulating monocytes are responsible for efficient crosspriming of CD8 + CTL after mucosal or skin immunization.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1074-7613eISSN: 1097-4180DOI: 10.1016/j.immuni.2006.01.005Titel-ID: cdi_hal_primary_oai_HAL_inserm_00000041v1
- Format
- –
- Schlagworte
- Adjuvants, Immunologic, Animals, CD8-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes - immunology, Cell Movement, Chemokine CCL20, Chemokines, Chemokines, CC, Chemokines, CC - metabolism, Chemokines, CC - physiology, Clodronic Acid, Clodronic Acid - pharmacology, Cytotoxicity, Dendritic Cells, Dendritic Cells - immunology, Dermis, Dermis - metabolism, Dinitrofluorobenzene, Dinitrofluorobenzene - pharmacology, Epithelium, Epithelium - immunology, Epithelium - metabolism, Female, H-2 Antigens, H-2 Antigens - genetics, H-2 Antigens - physiology, Immunization, Immunology, Langerhans Cells, Langerhans Cells - physiology, Life Sciences, Lymphocytes, Macrophage Inflammatory Proteins, Macrophage Inflammatory Proteins - metabolism, Macrophage Inflammatory Proteins - physiology, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Mouth Mucosa - cytology, Mouth Mucosa - immunology, Mouth Mucosa - metabolism, Nucleoproteins - immunology, Receptors, CCR6, Receptors, Chemokine - genetics, Receptors, Chemokine - physiology, Recruitment, Rodents, Spleen, Stem Cells - physiology