Details

Autor(en) / Beteiligte

• Jacquier, Hervé
• Assao, Bachir
• Chau, Françoise
• Guindo, Ousmane
• Condamine, Bénédicte
• Magnan, Mélanie
• Bridier-Nahmias, Antoine
• Sayingoza-Makombe, Nathan
• Moumouni, Aissatou
• Page, Anne-Laure
• Langendorf, Céline
• Coldiron, Matthew E.
• Denamur, Erick
• de Lastours, Victoire

Titel

Faecal carriage of extended-spectrum β-lactamase-producing Escherichia coli in a remote region of Niger

Ist Teil von

• The Journal of infection, 2023-09, Vol.87 (3), p.199-209

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Whole genome sequencing (WGS) of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E. coli) in developing countries is lacking. Here we describe the population structure and molecular characteristics of ESBL-E. coli faecal isolates in rural Southern Niger. Stools of 383 healthy participants were collected among which 92.4% were ESBL-Enterobacterales carriers. A subset of 90 ESBL-E. coli containing stools (109 ESBL-E. coli isolates) were further analysed by WGS, using short- and long-reads. Most isolates belonged to the commensalism-adapted phylogroup A (83.5%), with high clonal diversity. The blaCTX-M-15 gene was the major ESBL determinant (98.1%), chromosome-integrated in approximately 50% of cases, in multiple integration sites. When plasmid-borne, blaCTX-M-15 was found in IncF (57.4%) and IncY plasmids (26.2%). Closely related plasmids were found in different genetic backgrounds. Genomic environment analysis of blaCTX-M-15 in closely related strains argued for mobilisation between plasmids or from plasmid to chromosome. Massive prevalence of community faecal carriage of CTX-M-15-producing E. coli was observed in a rural region of Niger due to the spread of highly diverse A phylogroup commensalism-adapted clones, with frequent chromosomal integration of blaCTX-M-15. Plasmid spread was also observed. These data suggest a risk of sustainable implementation of ESBL in community faecal carriage. [Display omitted] ●Phylogroup A belonging of most of the ESBL-producing E. coli.●Chromosomal integration of the blaCTX-M-15 gene in 45% of the isolates.●Both clone and plasmid spread of the blaCTX-M-15 gene.●Highly conserved genetic environment of the blaCTX-M-15 gene suggesting its mobilisation.●WGS epidemiological studies on commensal and clinical E. coli in Africa warranted.