Journal of cell science, 2007-03, Vol.120 (Pt 5), p.838-848
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Mitochondrial bioenergetics and structural network organization
- Ist Teil von
- Journal of cell science, 2007-03, Vol.120 (Pt 5), p.838-848
- Ort / Verlag
- England: Company of Biologists
- Erscheinungsjahr
- 2007
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Mitochondria form a dynamic network, and it remains unclear how the alternate configurations interact with bioenergetics properties. The metabolic signals that link mitochondrial structure to its functional states have not been fully characterized. In this report, we analyze the bidirectional relationships between mitochondrial morphology and function in living human cells. First, we determined the effect of mitochondrial fission on energy production by using small interfering RNA (siRNA) targeting DRP1, which revealed the importance of membrane fluidity on the control of bioenergetics. Second, we followed the effect of rotenone, a specific inhibitor of respiratory chain complex I, which causes large structural perturbations, once a threshold was reached. Last, we followed changes in the mitochondrial network configuration in human cells that had been treated with modulators of oxidative phosphorylation, and in fibroblasts from two patients with mitochondrial disease where the respiratory rate, DeltaPsi and the generation of reactive oxygen species (ROS) were measured. Our data demonstrate that the relationship between mitochondrial network organization and bioenergetics is bidirectional, and we provide a model for analyzing the metabolic signals involved in this crosstalk.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9533eISSN: 1477-9137DOI: 10.1242/jcs.03381Titel-ID: cdi_hal_primary_oai_HAL_hal_04251082v1
- Format
- –
- Schlagworte
- Blotting, Western, Cell Line, Cell Survival - drug effects, Electron Transport Complex I - genetics, Electron Transport Complex I - metabolism, Electron Transport Complex IV - genetics, Electron Transport Complex IV - metabolism, Energy Metabolism - drug effects, Energy Metabolism - genetics, Energy Metabolism - physiology, Fibroblasts - cytology, Fibroblasts - drug effects, Fibroblasts - metabolism, GTP Phosphohydrolases - genetics, GTP Phosphohydrolases - metabolism, HeLa Cells, Humans, Life Sciences, Membrane Potential, Mitochondrial - drug effects, Microscopy, Fluorescence, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, Mitochondria - drug effects, Mitochondria - metabolism, Mitochondria - physiology, Mitochondrial Proteins - genetics, Mitochondrial Proteins - metabolism, Oxidation-Reduction - drug effects, Reactive Oxygen Species - metabolism, RNA Interference, Rotenone - pharmacology